Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 330764..331261 Replicon chromosome
Accession NZ_CP024245
Organism Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CQS47_RS01790 Protein ID WP_001173343.1
Coordinates 330764..330937 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CQS47_RS01795 Protein ID WP_000398619.1
Coordinates 330965..331261 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQS47_RS01780 327434..328891 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
CQS47_RS01785 329128..330645 + 1518 WP_001295074.1 lysine--tRNA ligase -
CQS47_RS01790 330764..330937 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CQS47_RS01795 330965..331261 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CQS47_RS01805 331488..331760 - 273 WP_000405647.1 N-acetyltransferase -
CQS47_RS01810 331772..332002 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
CQS47_RS01815 332183..333814 + 1632 WP_001216487.1 sensor histidine kinase -
CQS47_RS01820 333811..334530 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T87256 WP_001173343.1 NZ_CP024245:c330937-330764 [Escherichia coli O27:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T87256 NZ_CP024245:c330937-330764 [Escherichia coli O27:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT87256 WP_000398619.1 NZ_CP024245:c331261-330965 [Escherichia coli O27:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT87256 NZ_CP024245:c331261-330965 [Escherichia coli O27:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References