Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 140259..140851 Replicon plasmid unnamed
Accession NZ_CP024244
Organism Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag COG34_RS26760 Protein ID WP_000513531.1
Coordinates 140259..140507 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag COG34_RS26765 Protein ID WP_000681610.1
Coordinates 140504..140851 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG34_RS26715 135484..135714 + 231 WP_024220872.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
COG34_RS26720 135711..136127 + 417 WP_001356337.1 type II toxin-antitoxin system VapC family toxin -
COG34_RS26725 136094..136441 - 348 Protein_160 helix-turn-helix domain-containing protein -
COG34_RS26740 137995..138270 + 276 Protein_162 integrase core domain-containing protein -
COG34_RS26745 138279..138428 - 150 Protein_163 transposase -
COG34_RS26750 138744..139441 - 698 Protein_164 IS1 family transposase -
COG34_RS26755 139474..139794 + 321 WP_122993797.1 hypothetical protein -
COG34_RS26760 140259..140507 + 249 WP_000513531.1 type II toxin-antitoxin system HicA family toxin Toxin
COG34_RS26765 140504..140851 + 348 WP_000681610.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
COG34_RS26775 141078..142249 - 1172 Protein_168 IS91 family transposase -
COG34_RS26780 142249..142614 - 366 WP_000124139.1 hypothetical protein -
COG34_RS26790 142696..143298 + 603 Protein_170 DDE-type integrase/transposase/recombinase -
COG34_RS26795 143500..145311 + 1812 WP_000260520.1 two-partner secretion system transporter EtpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - cfaA / csaC / csaE / etpB / etpA 1..152012 152012
- inside IScluster/Tn - - 134184..143298 9114


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-80)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9298.86 Da        Isoelectric Point: 9.9409

>T87253 WP_000513531.1 NZ_CP024244:140259-140507 [Escherichia coli O128:H27]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKDMQGSRVRFFNAEINHTILMHCPHPESYIKGGTLKTIKQNLNEAG
AL

Download         Length: 249 bp

>T87253 NZ_CP024244:140259-140507 [Escherichia coli O128:H27]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTATT
TTCTTCTCTAGGATATGTCAAAAAAGACATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATTGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAACTATTAAACAGAACTTGAACGAGGCTGGG
GCTTTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13152.97 Da        Isoelectric Point: 4.9966

>AT87253 WP_000681610.1 NZ_CP024244:140504-140851 [Escherichia coli O128:H27]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAKTLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVGNHRAGA

Download         Length: 348 bp

>AT87253 NZ_CP024244:140504-140851 [Escherichia coli O128:H27]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAAAAACATTAGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGCCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCGCTGGAAAAAGA
AGTCGGTAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References