Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 68573..69120 Replicon plasmid unnamed
Accession NZ_CP024244
Organism Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag COG34_RS26285 Protein ID WP_000969996.1
Coordinates 68839..69120 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A2I2C2Y5
Locus tag COG34_RS26280 Protein ID WP_000079948.1
Coordinates 68573..68842 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG34_RS26235 64033..64599 + 567 WP_000469207.1 DUF2726 domain-containing protein -
COG34_RS26240 64596..65327 + 732 WP_001071488.1 type-F conjugative transfer system pilin acetylase TraX -
COG34_RS26245 65552..65734 - 183 WP_001393357.1 hypothetical protein -
COG34_RS26250 66241..66498 + 258 WP_000083823.1 replication regulatory protein RepA -
COG34_RS26260 66734..66808 + 75 WP_011387702.1 RepA leader peptide Tap -
COG34_RS26265 66801..67658 + 858 WP_000130978.1 incFII family plasmid replication initiator RepA -
COG34_RS26280 68573..68842 + 270 WP_000079948.1 hypothetical protein Antitoxin
COG34_RS26285 68839..69120 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
COG34_RS26290 69166..70014 + 849 WP_001057994.1 3'-5' exonuclease -
COG34_RS26295 70131..70613 + 483 WP_032182006.1 hypothetical protein -
COG34_RS26310 71148..71750 - 603 WP_000517689.1 hypothetical protein -
COG34_RS26320 72104..72736 + 633 WP_170866378.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - cfaA / csaC / csaE / etpB / etpA 1..152012 152012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T87251 WP_000969996.1 NZ_CP024244:68839-69120 [Escherichia coli O128:H27]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T87251 NZ_CP024244:68839-69120 [Escherichia coli O128:H27]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10251.64 Da        Isoelectric Point: 10.1734

>AT87251 WP_000079948.1 NZ_CP024244:68573-68842 [Escherichia coli O128:H27]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT87251 NZ_CP024244:68573-68842 [Escherichia coli O128:H27]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A2I2C2Y5

References