Detailed information of TA system TA087249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4772697..4773335
• Replicon: chromosome
• Accession: NZ_CP024243
• Organism: Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: COG34_RS24795
• Protein ID: WP_000813794.1
• Coordinates: 4773159..4773335 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: COG34_RS24790
• Protein ID: WP_001270286.1
• Coordinates: 4772697..4773113 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG34_RS24765	4768014..4769027	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
COG34_RS24770	4769045..4770190	-	1146	Protein_4497	ABC transporter substrate-binding protein	-
COG34_RS24780	4770325..4771022	+	698	Protein_4498	IS1 family transposase	-
COG34_RS24785	4771212..4772618	-	1407	WP_001421323.1	PLP-dependent aminotransferase family protein	-
COG34_RS24790	4772697..4773113	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
COG34_RS24795	4773159..4773335	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
COG34_RS24800	4773557..4773787	+	231	WP_000494244.1	YncJ family protein	-
COG34_RS24805	4773879..4775840	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
COG34_RS24810	4775913..4776449	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
COG34_RS24815	4776502..4777716	+	1215	WP_001349372.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4777756..4779021	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T87249 WP_000813794.1 NZ_CP024243:c4773335-4773159 [Escherichia coli O128:H27]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T87249 NZ_CP024243:c4773335-4773159 [Escherichia coli O128:H27]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT87249 WP_001270286.1 NZ_CP024243:c4773113-4772697 [Escherichia coli O128:H27]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT87249 NZ_CP024243:c4773113-4772697 [Escherichia coli O128:H27]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB