Detailed information of TA system TA087237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3498274..3498899
• Replicon: chromosome
• Accession: NZ_CP024243
• Organism: Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: COG34_RS18095
• Protein ID: WP_000911330.1
• Coordinates: 3498501..3498899 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: COG34_RS18090
• Protein ID: WP_000450524.1
• Coordinates: 3498274..3498501 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG34_RS18065	3494077..3494547	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
COG34_RS18070	3494547..3495119	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
COG34_RS18075	3495265..3496143	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
COG34_RS18080	3496160..3497194	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
COG34_RS18085	3497407..3498120	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
COG34_RS18090	3498274..3498501	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
COG34_RS18095	3498501..3498899	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
COG34_RS18100	3499046..3499909	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
COG34_RS18105	3499924..3501939	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
COG34_RS18110	3502013..3502711	+	699	WP_000679823.1	esterase	-
COG34_RS18115	3502821..3503021	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T87237 WP_000911330.1 NZ_CP024243:3498501-3498899 [Escherichia coli O128:H27]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T87237 NZ_CP024243:3498501-3498899 [Escherichia coli O128:H27]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT87237 WP_000450524.1 NZ_CP024243:3498274-3498501 [Escherichia coli O128:H27]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT87237 NZ_CP024243:3498274-3498501 [Escherichia coli O128:H27]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33