Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3203807..3204390 Replicon chromosome
Accession NZ_CP024243
Organism Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag COG34_RS16605 Protein ID WP_000254745.1
Coordinates 3204055..3204390 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag COG34_RS16600 Protein ID WP_000581937.1
Coordinates 3203807..3204055 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG34_RS16585 3199175..3200089 - 915 Protein_2991 HAMP domain-containing protein -
COG34_RS16590 3200146..3201447 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
COG34_RS16595 3201495..3203729 + 2235 WP_000226815.1 GTP pyrophosphokinase -
COG34_RS16600 3203807..3204055 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
COG34_RS16605 3204055..3204390 + 336 WP_000254745.1 endoribonuclease MazF Toxin
COG34_RS16610 3204461..3205252 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
COG34_RS16615 3205480..3207117 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
COG34_RS16620 3207205..3208503 + 1299 WP_000036723.1 phosphopyruvate hydratase -
COG34_RS16625 3208559..3208921 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T87236 WP_000254745.1 NZ_CP024243:3204055-3204390 [Escherichia coli O128:H27]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T87236 NZ_CP024243:3204055-3204390 [Escherichia coli O128:H27]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT87236 WP_000581937.1 NZ_CP024243:3203807-3204055 [Escherichia coli O128:H27]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT87236 NZ_CP024243:3203807-3204055 [Escherichia coli O128:H27]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References