Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2490369..2491169 Replicon chromosome
Accession NZ_CP024243
Organism Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag COG34_RS12800 Protein ID WP_000342449.1
Coordinates 2490642..2491169 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag COG34_RS12795 Protein ID WP_001277108.1
Coordinates 2490369..2490635 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG34_RS12775 2486027..2486695 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
COG34_RS12780 2486688..2487746 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
COG34_RS12785 2487991..2488845 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
COG34_RS12790 2489116..2490219 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
COG34_RS12795 2490369..2490635 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
COG34_RS12800 2490642..2491169 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
COG34_RS12805 2491166..2491549 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
COG34_RS12815 2491973..2493082 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
COG34_RS12820 2493130..2494056 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
COG34_RS12825 2494053..2495330 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
COG34_RS12830 2495327..2496094 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T87233 WP_000342449.1 NZ_CP024243:2490642-2491169 [Escherichia coli O128:H27]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T87233 NZ_CP024243:2490642-2491169 [Escherichia coli O128:H27]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT87233 WP_001277108.1 NZ_CP024243:2490369-2490635 [Escherichia coli O128:H27]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT87233 NZ_CP024243:2490369-2490635 [Escherichia coli O128:H27]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References