Detailed information of TA system TA087230

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1669310..1670144
• Replicon: chromosome
• Accession: NZ_CP024243
• Organism: Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: COG34_RS08755
• Protein ID: WP_074503007.1
• Coordinates: 1669767..1670144 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: COG34_RS08750
• Protein ID: WP_024183700.1
• Coordinates: 1669310..1669678 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG34_RS08710	1665035..1665919	+	885	WP_099588371.1	50S ribosome-binding GTPase	-
COG34_RS08715	1666038..1666715	+	678	WP_074504021.1	hypothetical protein	-
COG34_RS08720	1666721..1666954	+	234	WP_099588372.1	DUF905 family protein	-
COG34_RS08730	1667053..1667871	+	819	WP_074503010.1	DUF945 domain-containing protein	-
COG34_RS08735	1667963..1668448	+	486	WP_000206660.1	antirestriction protein	-
COG34_RS08740	1668464..1668940	+	477	WP_074503008.1	RadC family protein	-
COG34_RS08745	1669009..1669230	+	222	WP_000692295.1	DUF987 domain-containing protein	-
COG34_RS08750	1669310..1669678	+	369	WP_024183700.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
COG34_RS08755	1669767..1670144	+	378	WP_074503007.1	TA system toxin CbtA family protein	Toxin
COG34_RS08760	1670141..1670629	+	489	WP_000777667.1	hypothetical protein	-
COG34_RS08765	1670641..1670838	+	198	WP_000839291.1	DUF957 domain-containing protein	-
COG34_RS08770	1670923..1671687	+	765	Protein_1590	DUF4942 domain-containing protein	-
COG34_RS26920	1673012..1673125	+	114	Protein_1592	restriction endonuclease subunit M	-
COG34_RS08785	1673197..1673460	+	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
COG34_RS08790	1673457..1673783	+	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vipA/tssB	1650139..1670144	20005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14349.38 Da
• Isoelectric Point: 9.2442

>T87230 WP_074503007.1 NZ_CP024243:1669767-1670144 [Escherichia coli O128:H27]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T87230 NZ_CP024243:1669767-1670144 [Escherichia coli O128:H27]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTGTC
CCGCCTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCCCGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13617.41 Da
• Isoelectric Point: 7.7474

>AT87230 WP_024183700.1 NZ_CP024243:1669310-1669678 [Escherichia coli O128:H27]
VSDTFSGTTHPDDNRGRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 369 bp

>AT87230 NZ_CP024243:1669310-1669678 [Escherichia coli O128:H27]
GTGTCAGACACATTCTCCGGGACAACTCATCCCGACGACAATCGCGGCCGCCCCTGGTGGGGACTGCCCTGTACCGTGAC
ACCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAATTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA