Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1400819..1401395 Replicon chromosome
Accession NZ_CP024243
Organism Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag COG34_RS07270 Protein ID WP_001295743.1
Coordinates 1401108..1401395 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A6N8Q8R5
Locus tag COG34_RS07265 Protein ID WP_001422870.1
Coordinates 1400819..1401121 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG34_RS07250 1397415..1399565 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
COG34_RS07255 1399615..1399818 + 204 WP_000467859.1 YbdD/YjiX family protein -
COG34_RS07260 1399829..1400785 + 957 WP_001295745.1 GTPase -
COG34_RS07265 1400819..1401121 - 303 WP_001422870.1 BrnA antitoxin family protein Antitoxin
COG34_RS07270 1401108..1401395 - 288 WP_001295743.1 BrnT family toxin Toxin
COG34_RS07275 1401592..1402758 + 1167 WP_000800831.1 restriction endonuclease -
COG34_RS07280 1402822..1404441 + 1620 WP_001029745.1 type I restriction-modification system subunit M -
COG34_RS07285 1404431..1405735 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T87228 WP_001295743.1 NZ_CP024243:c1401395-1401108 [Escherichia coli O128:H27]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T87228 NZ_CP024243:c1401395-1401108 [Escherichia coli O128:H27]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11582.09 Da        Isoelectric Point: 9.4646

>AT87228 WP_001422870.1 NZ_CP024243:c1401121-1400819 [Escherichia coli O128:H27]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRSGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT87228 NZ_CP024243:c1401121-1400819 [Escherichia coli O128:H27]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGATCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A6N8Q8R5

References