Detailed information of TA system TA087218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4312981..4313818
• Replicon: chromosome
• Accession: NZ_CP024240
• Organism: Escherichia coli O114:H49 strain 90-9280

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: COG33_RS22515
• Protein ID: WP_000227784.1
• Coordinates: 4312981..4313523 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: COG33_RS22520
• Protein ID: WP_001297137.1
• Coordinates: 4313507..4313818 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG33_RS22495	4308520..4309431	-	912	WP_000705865.1	2-dehydropantoate 2-reductase	-
COG33_RS22500	4309599..4310090	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
COG33_RS22505	4310218..4311582	-	1365	WP_001000965.1	MFS transporter	-
COG33_RS22510	4311990..4312925	+	936	WP_001387350.1	sel1 repeat family protein	-
COG33_RS22515	4312981..4313523	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
COG33_RS22520	4313507..4313818	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
COG33_RS22525	4314003..4314893	-	891	WP_000971336.1	heme o synthase	-
COG33_RS22530	4314905..4315234	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
COG33_RS22535	4315234..4315848	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
COG33_RS22540	4315838..4317829	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
COG33_RS22545	4317851..4318798	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T87218 WP_000227784.1 NZ_CP024240:c4313523-4312981 [Escherichia coli O114:H49]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T87218 NZ_CP024240:c4313523-4312981 [Escherichia coli O114:H49]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT87218 WP_001297137.1 NZ_CP024240:c4313818-4313507 [Escherichia coli O114:H49]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT87218 NZ_CP024240:c4313818-4313507 [Escherichia coli O114:H49]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3