Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2127018..2127672 Replicon chromosome
Accession NZ_CP024240
Organism Escherichia coli O114:H49 strain 90-9280

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag COG33_RS11270 Protein ID WP_000244772.1
Coordinates 2127018..2127425 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag COG33_RS11275 Protein ID WP_000354046.1
Coordinates 2127406..2127672 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG33_RS11250 2122975..2124708 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
COG33_RS11255 2124714..2125424 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
COG33_RS11260 2125449..2126345 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
COG33_RS11265 2126457..2126978 + 522 WP_001055874.1 flavodoxin FldB -
COG33_RS11270 2127018..2127425 - 408 WP_000244772.1 protein YgfX Toxin
COG33_RS11275 2127406..2127672 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
COG33_RS11280 2127915..2128895 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
COG33_RS11285 2128972..2129631 - 660 WP_000250274.1 hemolysin III family protein -
COG33_RS11290 2129795..2130106 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
COG33_RS11295 2130151..2131584 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
COG33_RS11300 2131641..2132384 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T87210 WP_000244772.1 NZ_CP024240:c2127425-2127018 [Escherichia coli O114:H49]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T87210 NZ_CP024240:c2127425-2127018 [Escherichia coli O114:H49]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT87210 WP_000354046.1 NZ_CP024240:c2127672-2127406 [Escherichia coli O114:H49]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT87210 NZ_CP024240:c2127672-2127406 [Escherichia coli O114:H49]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References