Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1974978..1975561 Replicon chromosome
Accession NZ_CP024240
Organism Escherichia coli O114:H49 strain 90-9280

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag COG33_RS10535 Protein ID WP_000254745.1
Coordinates 1974978..1975313 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag COG33_RS10540 Protein ID WP_000581937.1
Coordinates 1975313..1975561 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG33_RS10520 1970865..1972163 - 1299 WP_000036723.1 phosphopyruvate hydratase -
COG33_RS10525 1972251..1973888 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
COG33_RS10530 1974116..1974907 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
COG33_RS10535 1974978..1975313 - 336 WP_000254745.1 endoribonuclease MazF Toxin
COG33_RS10540 1975313..1975561 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
COG33_RS10545 1975639..1977873 - 2235 WP_000226815.1 GTP pyrophosphokinase -
COG33_RS10550 1977921..1979222 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T87209 WP_000254745.1 NZ_CP024240:c1975313-1974978 [Escherichia coli O114:H49]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T87209 NZ_CP024240:c1975313-1974978 [Escherichia coli O114:H49]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT87209 WP_000581937.1 NZ_CP024240:c1975561-1975313 [Escherichia coli O114:H49]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT87209 NZ_CP024240:c1975561-1975313 [Escherichia coli O114:H49]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References