Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1681566..1682191 Replicon chromosome
Accession NZ_CP024240
Organism Escherichia coli O114:H49 strain 90-9280

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag COG33_RS09055 Protein ID WP_000911329.1
Coordinates 1681566..1681964 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag COG33_RS09060 Protein ID WP_000450524.1
Coordinates 1681964..1682191 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG33_RS09035 1677473..1677673 + 201 WP_000383836.1 YpfN family protein -
COG33_RS09040 1677754..1678452 - 699 WP_000679812.1 esterase -
COG33_RS09045 1678526..1680541 - 2016 WP_001388162.1 tRNA cytosine(34) acetyltransferase TmcA -
COG33_RS09050 1680556..1681419 - 864 WP_001267498.1 neutral zinc metallopeptidase -
COG33_RS09055 1681566..1681964 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
COG33_RS09060 1681964..1682191 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
COG33_RS09065 1682346..1683059 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
COG33_RS09070 1683272..1684306 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
COG33_RS09075 1684323..1685201 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
COG33_RS09080 1685347..1685919 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
COG33_RS09085 1685919..1686389 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T87208 WP_000911329.1 NZ_CP024240:c1681964-1681566 [Escherichia coli O114:H49]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T87208 NZ_CP024240:c1681964-1681566 [Escherichia coli O114:H49]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT87208 WP_000450524.1 NZ_CP024240:c1682191-1681964 [Escherichia coli O114:H49]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT87208 NZ_CP024240:c1682191-1681964 [Escherichia coli O114:H49]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References