Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4134357..4134975 Replicon chromosome
Accession NZ_CP024239
Organism Escherichia coli O15:H11 strain 90-9272

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag COG31_RS23325 Protein ID WP_001291435.1
Coordinates 4134357..4134575 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag COG31_RS23330 Protein ID WP_000344800.1
Coordinates 4134601..4134975 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG31_RS23290 (4129645) 4129645..4130217 + 573 WP_000779831.1 YbaY family lipoprotein -
COG31_RS23295 (4130248) 4130248..4130559 - 312 WP_000409911.1 MGMT family protein -
COG31_RS23305 (4130938) 4130938..4131291 + 354 WP_000878140.1 DUF1428 domain-containing protein -
COG31_RS23310 (4131333) 4131333..4132883 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
COG31_RS23315 (4133047) 4133047..4133517 - 471 WP_000136192.1 YlaC family protein -
COG31_RS23320 (4133633) 4133633..4134185 - 553 Protein_3951 maltose O-acetyltransferase -
COG31_RS23325 (4134357) 4134357..4134575 - 219 WP_001291435.1 HHA domain-containing protein Toxin
COG31_RS23330 (4134601) 4134601..4134975 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
COG31_RS23335 (4135521) 4135521..4138670 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
COG31_RS23340 (4138693) 4138693..4139886 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87196 WP_001291435.1 NZ_CP024239:c4134575-4134357 [Escherichia coli O15:H11]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87196 NZ_CP024239:c4134575-4134357 [Escherichia coli O15:H11]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT87196 WP_000344800.1 NZ_CP024239:c4134975-4134601 [Escherichia coli O15:H11]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87196 NZ_CP024239:c4134975-4134601 [Escherichia coli O15:H11]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References