Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2395663..2396160 Replicon chromosome
Accession NZ_CP024239
Organism Escherichia coli O15:H11 strain 90-9272

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag COG31_RS14435 Protein ID WP_001173343.1
Coordinates 2395663..2395836 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag COG31_RS14440 Protein ID WP_000398619.1
Coordinates 2395864..2396160 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG31_RS14425 (2392333) 2392333..2393790 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
COG31_RS14430 (2394027) 2394027..2395544 + 1518 WP_001295074.1 lysine--tRNA ligase -
COG31_RS14435 (2395663) 2395663..2395836 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
COG31_RS14440 (2395864) 2395864..2396160 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
COG31_RS14450 (2396387) 2396387..2396659 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
COG31_RS14455 (2396671) 2396671..2396901 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
COG31_RS14460 (2397082) 2397082..2398713 + 1632 WP_001216477.1 sensor histidine kinase -
COG31_RS14465 (2398710) 2398710..2399429 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2365403..2396160 30757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T87190 WP_001173343.1 NZ_CP024239:c2395836-2395663 [Escherichia coli O15:H11]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T87190 NZ_CP024239:c2395836-2395663 [Escherichia coli O15:H11]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT87190 WP_000398619.1 NZ_CP024239:c2396160-2395864 [Escherichia coli O15:H11]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT87190 NZ_CP024239:c2396160-2395864 [Escherichia coli O15:H11]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References