Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2175292..2175868 Replicon chromosome
Accession NZ_CP024239
Organism Escherichia coli O15:H11 strain 90-9272

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag COG31_RS13240 Protein ID WP_001297643.1
Coordinates 2175581..2175868 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag COG31_RS13235 Protein ID WP_000063156.1
Coordinates 2175292..2175594 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG31_RS13220 (2171888) 2171888..2174038 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
COG31_RS13225 (2174088) 2174088..2174291 + 204 WP_000467859.1 YbdD/YjiX family protein -
COG31_RS13230 (2174302) 2174302..2175258 + 957 WP_001297640.1 GTPase -
COG31_RS13235 (2175292) 2175292..2175594 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
COG31_RS13240 (2175581) 2175581..2175868 - 288 WP_001297643.1 BrnT family toxin Toxin
COG31_RS13245 (2176067) 2176067..2176764 - 698 Protein_2102 IS1 family transposase -
COG31_RS13250 (2176821) 2176821..2176916 + 96 Protein_2103 dienelactone hydrolase family protein -
COG31_RS13255 (2177062) 2177062..2178294 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
COG31_RS13260 (2178335) 2178335..2179615 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T87189 WP_001297643.1 NZ_CP024239:c2175868-2175581 [Escherichia coli O15:H11]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T87189 NZ_CP024239:c2175868-2175581 [Escherichia coli O15:H11]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT87189 WP_000063156.1 NZ_CP024239:c2175594-2175292 [Escherichia coli O15:H11]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT87189 NZ_CP024239:c2175594-2175292 [Escherichia coli O15:H11]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References