Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1858455..1859109 Replicon chromosome
Accession NZ_CP024239
Organism Escherichia coli O15:H11 strain 90-9272

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag COG31_RS11610 Protein ID WP_000244777.1
Coordinates 1858455..1858862 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag COG31_RS11615 Protein ID WP_000354046.1
Coordinates 1858843..1859109 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG31_RS11590 (1854412) 1854412..1856145 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
COG31_RS11595 (1856151) 1856151..1856861 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
COG31_RS11600 (1856886) 1856886..1857782 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
COG31_RS11605 (1857894) 1857894..1858415 + 522 WP_099550745.1 flavodoxin FldB -
COG31_RS11610 (1858455) 1858455..1858862 - 408 WP_000244777.1 protein YgfX Toxin
COG31_RS11615 (1858843) 1858843..1859109 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
COG31_RS11620 (1859352) 1859352..1860332 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
COG31_RS11625 (1860528) 1860528..1861187 - 660 WP_000250274.1 hemolysin III family protein -
COG31_RS11630 (1861351) 1861351..1861662 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
COG31_RS11635 (1861707) 1861707..1863140 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
COG31_RS11640 (1863197) 1863197..1863940 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T87188 WP_000244777.1 NZ_CP024239:c1858862-1858455 [Escherichia coli O15:H11]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T87188 NZ_CP024239:c1858862-1858455 [Escherichia coli O15:H11]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT87188 WP_000354046.1 NZ_CP024239:c1859109-1858843 [Escherichia coli O15:H11]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT87188 NZ_CP024239:c1859109-1858843 [Escherichia coli O15:H11]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References