Detailed information of TA system TA087178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 329037..329675
• Replicon: chromosome
• Accession: NZ_CP024239
• Organism: Escherichia coli O15:H11 strain 90-9272

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: COG31_RS03770
• Protein ID: WP_000813794.1
• Coordinates: 329037..329213 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: COG31_RS03775
• Protein ID: WP_001270286.1
• Coordinates: 329259..329675 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG31_RS03750 (324656)	324656..325870	-	1215	WP_071597387.1	BenE family transporter YdcO	-
COG31_RS03755 (325923)	325923..326459	+	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
COG31_RS03760 (326532)	326532..328493	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
COG31_RS03765 (328585)	328585..328815	-	231	WP_000494244.1	YncJ family protein	-
COG31_RS03770 (329037)	329037..329213	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
COG31_RS03775 (329259)	329259..329675	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
COG31_RS03780 (329754)	329754..331160	+	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
COG31_RS03785 (331405)	331405..332550	+	1146	WP_099550671.1	ABC transporter substrate-binding protein	-
COG31_RS03790 (332568)	332568..333581	+	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
COG31_RS03795 (333582)	333582..334523	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	323351..324616	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T87178 WP_000813794.1 NZ_CP024239:329037-329213 [Escherichia coli O15:H11]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T87178 NZ_CP024239:329037-329213 [Escherichia coli O15:H11]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT87178 WP_001270286.1 NZ_CP024239:329259-329675 [Escherichia coli O15:H11]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT87178 NZ_CP024239:329259-329675 [Escherichia coli O15:H11]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB