Detailed information of TA system TA087175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 167496..168031
• Replicon: plasmid unnamed
• Accession: NZ_CP024238
• Organism: Escherichia coli O15:H11 strain 90-9272

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0Z6V5
• Locus tag: COG31_RS01165
• Protein ID: WP_000222769.1
• Coordinates: 167496..167783 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: COG31_RS01170
• Protein ID: WP_001132900.1
• Coordinates: 167780..168031 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG31_RS01145 (163117)	163117..163409	+	293	Protein_199	IS66 family insertion sequence element accessory protein TnpB	-
COG31_RS01150 (163429)	163429..165000	+	1572	WP_099550619.1	IS66 family transposase	-
COG31_RS01160 (165626)	165626..167200	-	1575	WP_000805966.1	AAA family ATPase	-
COG31_RS01165 (167496)	167496..167783	-	288	WP_000222769.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
COG31_RS01170 (167780)	167780..168031	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
COG31_RS01175 (168104)	168104..168295	-	192	WP_040029215.1	hypothetical protein	-
COG31_RS01190 (168995)	168995..169852	-	858	WP_129111592.1	incFII family plasmid replication initiator RepA	-
COG31_RS28115 (169845)	169845..169892	-	48	WP_077822033.1	hypothetical protein	-
COG31_RS28120 (169883)	169883..170119	+	237	WP_249399531.1	replication protein RepA	-
COG31_RS01205 (170155)	170155..170415	-	261	WP_000078109.1	replication regulatory protein RepA	-
COG31_RS01210 (170655)	170655..171245	-	591	WP_000766805.1	DUF2726 domain-containing protein	-
COG31_RS01215 (171284)	171284..171493	-	210	WP_040029213.1	Hha/YmoA family nucleoid-associated regulatory protein	-
COG31_RS01220 (171539)	171539..172000	-	462	WP_001233850.1	thermonuclease family protein	-
COG31_RS01225 (172246)	172246..172458	-	213	WP_001301409.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	etpA / etpB / espC / eltB / eltA / eltA / eltB / csvA	1..274465	274465

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11074.18 Da
• Isoelectric Point: 10.5832

>T87175 WP_000222769.1 NZ_CP024238:c167783-167496 [Escherichia coli O15:H11]
MTYTVKFRDDALKEWLKLDKVIQQQFAKKLKKCSENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDGMLIIAVIAVGK
RERSNVYDLASERMR

Nucleotide

• Length: 288 bp

>T87175 NZ_CP024238:c167783-167496 [Escherichia coli O15:H11]
ATGACTTATACGGTCAAATTCAGGGATGATGCGCTGAAAGAGTGGCTGAAGCTGGATAAAGTCATTCAACAGCAATTTGC
GAAAAAGCTAAAGAAGTGCAGTGAAAATCCGCACATCCCTTCAGCAAAACTGCGAGGGATAAAGGACTGCTACAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGGCATGTTAATTATTGCGGTTATCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATGATCTCGCAAGCGAGAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT87175 WP_001132900.1 NZ_CP024238:c168031-167780 [Escherichia coli O15:H11]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT87175 NZ_CP024238:c168031-167780 [Escherichia coli O15:H11]
ATGCCTAACATTATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAACCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATCCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: D0Z6V5

Antitoxin

• AlphaFold DB: V0VIP2