Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 76889..77414 Replicon plasmid unnamed5
Accession NZ_CP024237
Organism Escherichia coli O6:H16 strain 2014EL-1346-6

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CNZ63_RS28055 Protein ID WP_001159871.1
Coordinates 76889..77194 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CNZ63_RS28060 Protein ID WP_000813634.1
Coordinates 77196..77414 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNZ63_RS28030 73133..73837 - 705 WP_001119661.1 helix-turn-helix domain-containing protein -
CNZ63_RS28035 74084..74764 + 681 WP_000452091.1 EAL domain-containing protein -
CNZ63_RS28045 75204..75845 - 642 WP_000470631.1 recombinase family protein -
CNZ63_RS28050 76175..76888 - 714 WP_000016957.1 site-specific integrase -
CNZ63_RS28055 76889..77194 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CNZ63_RS28060 77196..77414 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CNZ63_RS28070 77859..78116 + 258 WP_000343092.1 hypothetical protein -
CNZ63_RS28075 78116..78646 + 531 WP_011387704.1 hypothetical protein -
CNZ63_RS28080 78890..79732 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
CNZ63_RS28085 79735..80823 + 1089 WP_004100285.1 putative hexosyltransferase CapU -
CNZ63_RS28090 80828..81778 + 951 WP_001699589.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - cs3 / eltB / eltA / etpA / etpB 1..152713 152713
- flank IS/Tn - - 75204..75845 641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T87171 WP_001159871.1 NZ_CP024237:c77194-76889 [Escherichia coli O6:H16]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87171 NZ_CP024237:c77194-76889 [Escherichia coli O6:H16]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT87171 WP_000813634.1 NZ_CP024237:c77414-77196 [Escherichia coli O6:H16]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87171 NZ_CP024237:c77414-77196 [Escherichia coli O6:H16]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References