Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 26555..27080 Replicon plasmid unnamed5
Accession NZ_CP024237
Organism Escherichia coli O6:H16 strain 2014EL-1346-6

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CNZ63_RS27670 Protein ID WP_001159871.1
Coordinates 26555..26860 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D7GK94
Locus tag CNZ63_RS27675 Protein ID WP_000829078.1
Coordinates 26862..27080 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNZ63_RS27645 21709..22838 + 1130 Protein_24 IS110 family transposase -
CNZ63_RS27650 23091..24321 - 1231 Protein_25 IS66 family transposase -
CNZ63_RS27655 24531..24839 - 309 WP_000030204.1 molecular chaperone GroEL -
CNZ63_RS27660 24926..25570 - 645 WP_001144037.1 ParA family protein -
CNZ63_RS27665 25748..26554 - 807 WP_000016960.1 site-specific integrase -
CNZ63_RS27670 26555..26860 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CNZ63_RS27675 26862..27080 - 219 WP_000829078.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CNZ63_RS27690 27602..27859 + 258 WP_000343095.1 hypothetical protein -
CNZ63_RS27695 27859..28401 + 543 WP_000194544.1 hypothetical protein -
CNZ63_RS27700 28645..29487 + 843 WP_162749921.1 polysaccharide deacetylase family protein -
CNZ63_RS27705 29490..30578 + 1089 WP_004100285.1 putative hexosyltransferase CapU -
CNZ63_RS27710 30583..31533 + 951 WP_001699589.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - cs3 / eltB / eltA / etpA / etpB 1..152713 152713


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T87170 WP_001159871.1 NZ_CP024237:c26860-26555 [Escherichia coli O6:H16]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87170 NZ_CP024237:c26860-26555 [Escherichia coli O6:H16]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8440.51 Da        Isoelectric Point: 6.3212

>AT87170 WP_000829078.1 NZ_CP024237:c27080-26862 [Escherichia coli O6:H16]
MKRRITVTVDSDSYQLLKAYDVNISGLVSTIMQNEARRLRAERWKAENREGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87170 NZ_CP024237:c27080-26862 [Escherichia coli O6:H16]
ATGAAACGGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCATCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A7ZGP5

References