Detailed information of TA system TA087154

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2944331..2945165
• Replicon: chromosome
• Accession: NZ_CP024232
• Organism: Escherichia coli O6:H16 strain 2014EL-1346-6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CNZ63_RS15325
• Protein ID: WP_140159973.1
• Coordinates: 2944331..2944699 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CNZ63_RS15330
• Protein ID: WP_001285580.1
• Coordinates: 2944797..2945165 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNZ63_RS15300	2939458..2940606	-	1149	WP_000905925.1	capsule polysaccharide transporter	-
CNZ63_RS15305	2940678..2941661	-	984	WP_001355482.1	KpsF/GutQ family sugar-phosphate isomerase	-
CNZ63_RS15310	2942472..2942642	-	171	Protein_2756	IS110 family transposase	-
CNZ63_RS15315	2942985..2943827	-	843	WP_001280493.1	DUF4942 domain-containing protein	-
CNZ63_RS15320	2943912..2944109	-	198	WP_023563519.1	DUF957 domain-containing protein	-
CNZ63_RS28730	2944185..2944334	-	150	Protein_2759	hypothetical protein	-
CNZ63_RS15325	2944331..2944699	-	369	WP_140159973.1	TA system toxin CbtA family protein	Toxin
CNZ63_RS15330	2944797..2945165	-	369	WP_001285580.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CNZ63_RS15335	2945244..2945465	-	222	WP_000691818.1	DUF987 domain-containing protein	-
CNZ63_RS15345	2945602..2945781	-	180	WP_001398324.1	hypothetical protein	-
CNZ63_RS15350	2945823..2946520	+	698	Protein_2764	IS1 family transposase	-
CNZ63_RS15360	2947329..2947553	+	225	WP_001395295.1	transposase	-
CNZ63_RS15365	2947555..2948334	-	780	Protein_2766	IS3 family transposase	-
CNZ63_RS15370	2948501..2949469	-	969	WP_023486738.1	IS5 family transposase	-
CNZ63_RS15375	2949502..2949615	+	114	Protein_2768	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsM / kpsT / kpsC / kpsU / kpsD / kpsE / kpsF	2923417..2951125	27708

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (12-109)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13616.51 Da
• Isoelectric Point: 9.1652

>T87154 WP_140159973.1 NZ_CP024232:c2944699-2944331 [Escherichia coli O6:H16]
ITQHTRTGGIARPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVSFLVEKYALVRTDQPGFSAC
TRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 369 bp

>T87154 NZ_CP024232:c2944699-2944331 [Escherichia coli O6:H16]
ATTACCCAACACACACGTACGGGAGGCATCGCGCGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTCCCGGTTGCT
GGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTGAAGCTGGCA
TTTCACTGTGTGATGCGGTGAGCTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTCAGCGCCTGT
ACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGACAACTACAG
AACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13670.43 Da
• Isoelectric Point: 6.9462

>AT87154 WP_001285580.1 NZ_CP024232:c2945165-2944797 [Escherichia coli O6:H16]
VSDTLPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADSYHLNQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT87154 NZ_CP024232:c2945165-2944797 [Escherichia coli O6:H16]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGAACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA