Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1629328..1629825 Replicon chromosome
Accession NZ_CP024232
Organism Escherichia coli O6:H16 strain 2014EL-1346-6

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CNZ63_RS08560 Protein ID WP_001173343.1
Coordinates 1629328..1629501 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag CNZ63_RS08565 Protein ID WP_071597404.1
Coordinates 1629529..1629825 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNZ63_RS08550 1625993..1627450 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
CNZ63_RS08555 1627687..1629204 + 1518 WP_001295074.1 lysine--tRNA ligase -
CNZ63_RS08560 1629328..1629501 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CNZ63_RS08565 1629529..1629825 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CNZ63_RS08575 1630052..1630324 - 273 WP_000405647.1 N-acetyltransferase -
CNZ63_RS08580 1630336..1630566 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
CNZ63_RS08585 1630747..1632378 + 1632 WP_001216490.1 sensor histidine kinase -
CNZ63_RS08590 1632375..1633094 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T87149 WP_001173343.1 NZ_CP024232:c1629501-1629328 [Escherichia coli O6:H16]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T87149 NZ_CP024232:c1629501-1629328 [Escherichia coli O6:H16]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT87149 WP_071597404.1 NZ_CP024232:c1629825-1629529 [Escherichia coli O6:H16]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT87149 NZ_CP024232:c1629825-1629529 [Escherichia coli O6:H16]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References