Detailed information of TA system TA087145

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 320187..321031
• Replicon: chromosome
• Accession: NZ_CP024232
• Organism: Escherichia coli O6:H16 strain 2014EL-1346-6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: CNZ63_RS01680
• Protein ID: WP_000854686.1
• Coordinates: 320187..320570 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: CNZ63_RS01685
• Protein ID: WP_001285602.1
• Coordinates: 320651..321031 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNZ63_RS01650	316864..317568	+	705	WP_001241677.1	leucyl/phenylalanyl-tRNA--protein transferase	-
CNZ63_RS01655	317853..318071	+	219	WP_001040187.1	translation initiation factor IF-1	-
CNZ63_RS01665	318561..319403	-	843	WP_001529751.1	DUF4942 domain-containing protein	-
CNZ63_RS01670	319488..319685	-	198	WP_000839253.1	DUF957 domain-containing protein	-
CNZ63_RS01675	319702..320190	-	489	WP_001054233.1	hypothetical protein	-
CNZ63_RS01680	320187..320570	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
CNZ63_RS01685	320651..321031	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CNZ63_RS01690	321042..321419	-	378	WP_000271247.1	hypothetical protein	-
CNZ63_RS01695	321592..322704	-	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
CNZ63_RS01700	322772..323074	-	303	Protein_309	hypothetical protein	-
CNZ63_RS01705	323093..323314	-	222	WP_000692298.1	DUF987 domain-containing protein	-
CNZ63_RS01710	323377..323853	-	477	WP_001186726.1	RadC family protein	-
CNZ63_RS01715	323869..324354	-	486	WP_000214307.1	antirestriction protein	-
CNZ63_RS01720	324446..325264	-	819	WP_001234726.1	DUF945 domain-containing protein	-
CNZ63_RS01730	325364..325597	-	234	WP_001119717.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T87145 WP_000854686.1 NZ_CP024232:c320570-320187 [Escherichia coli O6:H16]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T87145 NZ_CP024232:c320570-320187 [Escherichia coli O6:H16]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT87145 WP_001285602.1 NZ_CP024232:c321031-320651 [Escherichia coli O6:H16]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT87145 NZ_CP024232:c321031-320651 [Escherichia coli O6:H16]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG