Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 19800..20347 Replicon plasmid unnamed2
Accession NZ_CP024230
Organism Escherichia coli O25:NM strain 2014EL-1343-2

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag A3411_RS25705 Protein ID WP_000970007.1
Coordinates 19800..20081 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag A3411_RS25710 Protein ID WP_000079941.1
Coordinates 20078..20347 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3411_RS25665 15505..16072 - 568 Protein_15 hypothetical protein -
A3411_RS25675 16426..17028 + 603 WP_000517695.1 hypothetical protein -
A3411_RS25680 17085..17579 + 495 WP_001459505.1 hypothetical protein -
A3411_RS27005 17579..17845 + 267 WP_000955263.1 hypothetical protein -
A3411_RS25695 18322..18795 - 474 WP_001334658.1 hypothetical protein -
A3411_RS25700 18912..19760 - 849 WP_001057996.1 3'-5' exonuclease -
A3411_RS25705 19800..20081 - 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A3411_RS25710 20078..20347 - 270 WP_000079941.1 hypothetical protein Antitoxin
A3411_RS25725 21257..22288 - 1032 WP_000907882.1 replication initiation protein -
A3411_RS25740 22991..23632 + 642 WP_000335851.1 transcription termination factor NusG -
A3411_RS25745 23773..24438 + 666 WP_000154140.1 hypothetical protein -
A3411_RS25755 24709..24939 + 231 WP_071596634.1 PilI type IV pilus biogenesis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrS1 - 1..73915 73915


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T87140 WP_000970007.1 NZ_CP024230:c20081-19800 [Escherichia coli O25:NM]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T87140 NZ_CP024230:c20081-19800 [Escherichia coli O25:NM]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT87140 WP_000079941.1 NZ_CP024230:c20347-20078 [Escherichia coli O25:NM]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT87140 NZ_CP024230:c20347-20078 [Escherichia coli O25:NM]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References