Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3989877..3990531 Replicon chromosome
Accession NZ_CP024228
Organism Escherichia coli O25:NM strain 2014EL-1343-2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag A3411_RS21010 Protein ID WP_000244781.1
Coordinates 3990124..3990531 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A3411_RS21005 Protein ID WP_000354046.1
Coordinates 3989877..3990143 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3411_RS20985 3985965..3987398 - 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -
A3411_RS20990 3987443..3987754 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
A3411_RS20995 3987918..3988577 + 660 WP_000250274.1 hemolysin III family protein -
A3411_RS21000 3988654..3989634 - 981 WP_000886087.1 tRNA-modifying protein YgfZ -
A3411_RS21005 3989877..3990143 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A3411_RS21010 3990124..3990531 + 408 WP_000244781.1 protein YgfX Toxin
A3411_RS21015 3990571..3991092 - 522 WP_001055874.1 flavodoxin FldB -
A3411_RS21020 3991204..3992100 + 897 WP_001735667.1 site-specific tyrosine recombinase XerD -
A3411_RS21025 3992125..3992835 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A3411_RS21030 3992841..3994574 + 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T87134 WP_000244781.1 NZ_CP024228:3990124-3990531 [Escherichia coli O25:NM]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T87134 NZ_CP024228:3990124-3990531 [Escherichia coli O25:NM]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT87134 WP_000354046.1 NZ_CP024228:3989877-3990143 [Escherichia coli O25:NM]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT87134 NZ_CP024228:3989877-3990143 [Escherichia coli O25:NM]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References