Detailed information of TA system TA087133

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3895583..3896415
• Replicon: chromosome
• Accession: NZ_CP024228
• Organism: Escherichia coli O25:NM strain 2014EL-1343-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: A3411_RS20435
• Protein ID: WP_000854765.1
• Coordinates: 3895583..3895957 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: A3411_RS20440
• Protein ID: WP_001295723.1
• Coordinates: 3896047..3896415 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3411_RS20415	3893375..3894595	+	1221	WP_001735713.1	capsular biosynthesis protein	-
A3411_RS20420	3894618..3894854	-	237	WP_001735712.1	hypothetical protein	-
A3411_RS20425	3894957..3895133	-	177	WP_000839288.1	DUF957 domain-containing protein	-
A3411_RS20430	3895150..3895586	-	437	Protein_3711	hypothetical protein	-
A3411_RS20435	3895583..3895957	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
A3411_RS20440	3896047..3896415	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A3411_RS20445	3896578..3896799	-	222	WP_001735710.1	DUF987 domain-containing protein	-
A3411_RS20450	3896868..3897344	-	477	WP_001186725.1	RadC family protein	-
A3411_RS20455	3897360..3897845	-	486	WP_001735709.1	antirestriction protein	-
A3411_RS20460	3897900..3898718	-	819	WP_074481115.1	DUF945 domain-containing protein	-
A3411_RS20470	3898873..3899031	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T87133 WP_000854765.1 NZ_CP024228:c3895957-3895583 [Escherichia coli O25:NM]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T87133 NZ_CP024228:c3895957-3895583 [Escherichia coli O25:NM]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT87133 WP_001295723.1 NZ_CP024228:c3896415-3896047 [Escherichia coli O25:NM]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT87133 NZ_CP024228:c3896415-3896047 [Escherichia coli O25:NM]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52