Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2304243..2304819 Replicon chromosome
Accession NZ_CP024228
Organism Escherichia coli O25:NM strain 2014EL-1343-2

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag A3411_RS12215 Protein ID WP_001297643.1
Coordinates 2304532..2304819 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag A3411_RS12210 Protein ID WP_000063156.1
Coordinates 2304243..2304545 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3411_RS12195 2300839..2302989 + 2151 WP_001736090.1 pyruvate/proton symporter BtsT -
A3411_RS12200 2303039..2303242 + 204 WP_000467859.1 YbdD/YjiX family protein -
A3411_RS12205 2303253..2304209 + 957 WP_099582884.1 GTPase -
A3411_RS12210 2304243..2304545 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
A3411_RS12215 2304532..2304819 - 288 WP_001297643.1 BrnT family toxin Toxin
A3411_RS12220 2305018..2305715 - 698 Protein_2232 IS1 family transposase -
A3411_RS12225 2305772..2305867 + 96 Protein_2233 dienelactone hydrolase family protein -
A3411_RS12230 2306013..2307245 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
A3411_RS12235 2307286..2308566 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T87130 WP_001297643.1 NZ_CP024228:c2304819-2304532 [Escherichia coli O25:NM]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T87130 NZ_CP024228:c2304819-2304532 [Escherichia coli O25:NM]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT87130 WP_000063156.1 NZ_CP024228:c2304545-2304243 [Escherichia coli O25:NM]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT87130 NZ_CP024228:c2304545-2304243 [Escherichia coli O25:NM]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References