Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 569608..570246 Replicon chromosome
Accession NZ_CP024228
Organism Escherichia coli O25:NM strain 2014EL-1343-2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A3411_RS02995 Protein ID WP_000813794.1
Coordinates 569608..569784 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A3411_RS03000 Protein ID WP_001270286.1
Coordinates 569830..570246 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3411_RS02975 565227..566441 - 1215 WP_099582830.1 BenE family transporter YdcO -
A3411_RS02980 566494..567030 + 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
A3411_RS02985 567103..569064 + 1962 WP_001735216.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A3411_RS02990 569156..569386 - 231 WP_000494244.1 YncJ family protein -
A3411_RS02995 569608..569784 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A3411_RS03000 569830..570246 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A3411_RS03005 570325..571731 + 1407 WP_001735217.1 PLP-dependent aminotransferase family protein -
A3411_RS03010 571976..573121 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
A3411_RS03015 573139..574152 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
A3411_RS03020 574153..575094 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T87125 WP_000813794.1 NZ_CP024228:569608-569784 [Escherichia coli O25:NM]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T87125 NZ_CP024228:569608-569784 [Escherichia coli O25:NM]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT87125 WP_001270286.1 NZ_CP024228:569830-570246 [Escherichia coli O25:NM]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT87125 NZ_CP024228:569830-570246 [Escherichia coli O25:NM]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References