Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 60897..61471 Replicon plasmid unnamed4
Accession NZ_CP024227
Organism Escherichia coli O169:H41 strain 2014EL-1345-2

Toxin (Protein)


Gene name relE Uniprot ID A0A0S3PMZ8
Locus tag CJV63_RS26435 Protein ID WP_001595119.1
Coordinates 60897..61223 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0S3PN98
Locus tag CJV63_RS26440 Protein ID WP_001595120.1
Coordinates 61220..61471 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJV63_RS26410 57470..58447 - 978 WP_023486339.1 RepB family plasmid replication initiator protein -
CJV63_RS26425 59320..60497 - 1178 Protein_71 IS91 family transposase -
CJV63_RS26430 60497..60793 - 297 WP_000766063.1 hypothetical protein -
CJV63_RS26435 60897..61223 - 327 WP_001595119.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CJV63_RS26440 61220..61471 - 252 WP_001595120.1 hypothetical protein Antitoxin
CJV63_RS26445 62025..62891 + 867 WP_001595122.1 AAA family ATPase -
CJV63_RS26450 62891..63922 + 1032 WP_001595123.1 ParB/RepB/Spo0J family partition protein -
CJV63_RS26455 63946..64359 + 414 WP_032159708.1 hypothetical protein -
CJV63_RS27495 64356..64679 + 324 WP_000143877.1 hypothetical protein -
CJV63_RS26465 64718..65992 - 1275 WP_001595125.1 Y-family DNA polymerase -
CJV63_RS26470 65992..66414 - 423 WP_001595126.1 translesion error-prone DNA polymerase V autoproteolytic subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - faeE / faeD / cofA / cssA / estIa 1..145086 145086
- flank IS/Tn - - 59574..60497 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12187.44 Da        Isoelectric Point: 10.4373

>T87113 WP_001595119.1 NZ_CP024227:c61223-60897 [Escherichia coli O169:H41]
MTYTVKFRDDALKEWLKLDKAIQQQFAKKLKKCSENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAIGK
RERSDVYNLASRVGSLAAAACHGLKLPL

Download         Length: 327 bp

>T87113 NZ_CP024227:c61223-60897 [Escherichia coli O169:H41]
ATGACTTACACAGTCAAATTCAGGGATGATGCACTGAAAGAATGGCTGAAACTGGATAAGGCCATTCAACAGCAATTTGC
TAAAAAGCTAAAGAAGTGCAGTGAAAATCCTCATATTCCCTCTGCTAAGCTGCGCGGGATAAAAGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATAGATGATCAATTAATTATCGCTGTTGTTGCTATTGGGAAA
CGTGAACGTAGTGATGTTTATAATCTTGCCAGCCGAGTAGGCAGCCTGGCGGCTGCGGCTTGTCATGGACTGAAATTACC
GTTATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9144.43 Da        Isoelectric Point: 4.2975

>AT87113 WP_001595120.1 NZ_CP024227:c61471-61220 [Escherichia coli O169:H41]
MPNIILTDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYGKMLDAMDDQELVKLVAERSNQKLHDVDLD
SYL

Download         Length: 252 bp

>AT87113 NZ_CP024227:c61471-61220 [Escherichia coli O169:H41]
ATGCCTAACATCATTCTTACCGACACAAGCGCCAGTGTAAGCGAACTTAAGAAAAACCCGATGGCTACAGTCAGCGCTGG
TGATGGCTACCCAGTTGCTATTCTGAACCGCAACCAGCCAGCCTTTTACTGCGTACCCGCAGAGTTGTACGGAAAGATGC
TCGATGCCATGGACGATCAGGAACTGGTTAAGCTAGTCGCCGAACGCAGCAACCAGAAGCTGCACGATGTGGATCTGGAT
AGCTACTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S3PMZ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3PN98

References