Detailed information of TA system TA087107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 22388..22935
• Replicon: plasmid unnamed2
• Accession: NZ_CP024225
• Organism: Escherichia coli O169:H41 strain 2014EL-1345-2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CJV63_RS25430
• Protein ID: WP_000969991.1
• Coordinates: 22388..22669 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6G9U792
• Locus tag: CJV63_RS25435
• Protein ID: WP_000079935.1
• Coordinates: 22666..22935 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJV63_RS25380	17513..17641	-	129	Protein_27	DUF2164 family protein	-
CJV63_RS25390	17901..18002	-	102	Protein_28	methionyl-tRNA formyltransferase	-
CJV63_RS25395	18017..18526	-	510	WP_000115002.1	peptide deformylase	-
CJV63_RS25405	19054..19419	+	366	WP_000124093.1	hypothetical protein	-
CJV63_RS25410	19419..20535	+	1117	Protein_31	IS91 family transposase	-
CJV63_RS25420	20895..21377	-	483	WP_001311056.1	hypothetical protein	-
CJV63_RS25425	21494..22342	-	849	WP_001058003.1	3'-5' exonuclease	-
CJV63_RS25430	22388..22669	-	282	WP_000969991.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CJV63_RS25435	22666..22935	-	270	WP_000079935.1	hypothetical protein	Antitoxin
CJV63_RS25450	23853..24875	-	1023	WP_032268691.1	replication initiation protein	-
CJV63_RS25455	24868..24942	-	75	WP_071703955.1	RepA leader peptide Tap	-
CJV63_RS25465	25178..25435	-	258	WP_001404371.1	replication regulatory protein RepA	-
CJV63_RS25475	25991..26185	-	195	WP_000818784.1	hypothetical protein	-
CJV63_RS25485	27670..27920	-	251	Protein_40	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..27947	27947
	-	flank	IS/Tn	-	-	19782..20504	722

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10860.56 Da
• Isoelectric Point: 8.4615

>T87107 WP_000969991.1 NZ_CP024225:c22669-22388 [Escherichia coli O169:H41]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASSLAGLPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T87107 NZ_CP024225:c22669-22388 [Escherichia coli O169:H41]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCTGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10198.53 Da
• Isoelectric Point: 8.8735

>AT87107 WP_000079935.1 NZ_CP024225:c22935-22666 [Escherichia coli O169:H41]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLTHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT87107 NZ_CP024225:c22935-22666 [Escherichia coli O169:H41]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTACCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6G9U792