Detailed information of TA system TA087105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4696023..4696661
• Replicon: chromosome
• Accession: NZ_CP024223
• Organism: Escherichia coli O169:H41 strain 2014EL-1345-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3T4J978
• Locus tag: CJV63_RS23825
• Protein ID: WP_001612987.1
• Coordinates: 4696023..4696199 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CJV63_RS23830
• Protein ID: WP_001270286.1
• Coordinates: 4696245..4696661 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJV63_RS23805	4691642..4692856	-	1215	WP_072148478.1	BenE family transporter YdcO	-
CJV63_RS23810	4692909..4693445	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
CJV63_RS23815	4693518..4695479	+	1962	WP_001718255.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CJV63_RS23820	4695571..4695801	-	231	WP_000494244.1	YncJ family protein	-
CJV63_RS23825	4696023..4696199	+	177	WP_001612987.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CJV63_RS23830	4696245..4696661	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CJV63_RS23835	4696740..4698146	+	1407	WP_001718258.1	PLP-dependent aminotransferase family protein	-
CJV63_RS23840	4698391..4699536	+	1146	WP_001612991.1	ABC transporter substrate-binding protein	-
CJV63_RS23845	4699554..4700567	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
CJV63_RS23850	4700568..4701509	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4690454..4691602	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6725.78 Da
• Isoelectric Point: 10.9223

>T87105 WP_001612987.1 NZ_CP024223:4696023-4696199 [Escherichia coli O169:H41]
VKQSEFRRWLESQGVDVANGSNHLKVRFHGMRSVMPRHPCDEIKEPLRKAIQKQLGLS

Nucleotide

• Length: 177 bp

>T87105 NZ_CP024223:4696023-4696199 [Escherichia coli O169:H41]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAAGTCAG
GTTTCATGGGATGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCAGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT87105 WP_001270286.1 NZ_CP024223:4696245-4696661 [Escherichia coli O169:H41]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT87105 NZ_CP024223:4696245-4696661 [Escherichia coli O169:H41]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGAGAATTGGCAAACCTAAACAGGAAAT
TACTCGTTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A3T4J978