Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1930933..1931564 Replicon chromosome
Accession NZ_CP024223
Organism Escherichia coli O169:H41 strain 2014EL-1345-2

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag CJV63_RS09870 Protein ID WP_001259385.1
Coordinates 1931289..1931564 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CJV63_RS09865 Protein ID WP_000593555.1
Coordinates 1930933..1931292 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJV63_RS09840 1927065..1928009 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
CJV63_RS09845 1928006..1928839 + 834 WP_099490863.1 nickel ABC transporter permease subunit NikC -
CJV63_RS09850 1928839..1929603 + 765 WP_001614016.1 nickel import ATP-binding protein NikD -
CJV63_RS09855 1929600..1930406 + 807 WP_001614017.1 nickel import ATP-binding protein NikE -
CJV63_RS09860 1930412..1930813 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CJV63_RS09865 1930933..1931292 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CJV63_RS09870 1931289..1931564 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
CJV63_RS09875 1931637..1932761 - 1125 WP_001314210.1 ABC transporter permease -
CJV63_RS09880 1932761..1935496 - 2736 WP_001614021.1 ribosome-associated ATPase/putative transporter RbbA -
CJV63_RS09885 1935493..1936560 - 1068 WP_001614022.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T87100 WP_001259385.1 NZ_CP024223:c1931564-1931289 [Escherichia coli O169:H41]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T87100 NZ_CP024223:c1931564-1931289 [Escherichia coli O169:H41]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT87100 WP_000593555.1 NZ_CP024223:c1931292-1930933 [Escherichia coli O169:H41]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT87100 NZ_CP024223:c1931292-1930933 [Escherichia coli O169:H41]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References