Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1909426..1910226 Replicon chromosome
Accession NZ_CP024223
Organism Escherichia coli O169:H41 strain 2014EL-1345-2

Toxin (Protein)


Gene name ataT Uniprot ID A0A0K4AFE4
Locus tag CJV63_RS09745 Protein ID WP_001614005.1
Coordinates 1909426..1909953 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag CJV63_RS09750 Protein ID WP_001277108.1
Coordinates 1909960..1910226 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJV63_RS09715 1904501..1905268 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
CJV63_RS09720 1905265..1906542 - 1278 WP_001614002.1 branched chain amino acid ABC transporter permease LivM -
CJV63_RS09725 1906539..1907465 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CJV63_RS09730 1907513..1908622 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
CJV63_RS09740 1909046..1909429 + 384 WP_001614003.1 aspartate 1-decarboxylase autocleavage activator PanM -
CJV63_RS09745 1909426..1909953 - 528 WP_001614005.1 GNAT family N-acetyltransferase Toxin
CJV63_RS09750 1909960..1910226 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
CJV63_RS09755 1910376..1911479 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
CJV63_RS09760 1911750..1912604 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
CJV63_RS09765 1912849..1913907 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
CJV63_RS09770 1913900..1914568 - 669 WP_001718990.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19676.62 Da        Isoelectric Point: 7.3502

>T87099 WP_001614005.1 NZ_CP024223:c1909953-1909426 [Escherichia coli O169:H41]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T87099 NZ_CP024223:c1909953-1909426 [Escherichia coli O169:H41]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT87099 WP_001277108.1 NZ_CP024223:c1910226-1909960 [Escherichia coli O169:H41]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT87099 NZ_CP024223:c1910226-1909960 [Escherichia coli O169:H41]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4AFE4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References