Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 498395..499032 Replicon chromosome
Accession NZ_CP024203
Organism Bacillus velezensis strain NKG-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CS376_RS02555 Protein ID WP_003156187.1
Coordinates 498682..499032 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag CS376_RS02550 Protein ID WP_003156188.1
Coordinates 498395..498676 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS376_RS02530 494760..495359 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
CS376_RS02535 495452..495817 + 366 WP_094031521.1 holo-ACP synthase -
CS376_RS02540 495982..496989 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
CS376_RS02545 497106..498275 + 1170 WP_025284272.1 alanine racemase -
CS376_RS02550 498395..498676 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CS376_RS02555 498682..499032 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CS376_RS02560 499150..499971 + 822 WP_003156182.1 STAS domain-containing protein -
CS376_RS02565 499976..500341 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
CS376_RS02570 500344..500745 + 402 WP_099566419.1 anti-sigma regulatory factor -
CS376_RS02575 500757..501764 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
CS376_RS02580 501828..502157 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
CS376_RS02585 502154..502636 + 483 WP_007609591.1 anti-sigma B factor RsbW -
CS376_RS02590 502602..503390 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
CS376_RS02595 503390..503992 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T87087 WP_003156187.1 NZ_CP024203:498682-499032 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T87087 NZ_CP024203:498682-499032 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT87087 WP_003156188.1 NZ_CP024203:498395-498676 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT87087 NZ_CP024203:498395-498676 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References