Detailed information of TA system TA087087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 498395..499032
• Replicon: chromosome
• Accession: NZ_CP024203
• Organism: Bacillus velezensis strain NKG-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CS376_RS02555
• Protein ID: WP_003156187.1
• Coordinates: 498682..499032 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CS376_RS02550
• Protein ID: WP_003156188.1
• Coordinates: 498395..498676 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS376_RS02530	494760..495359	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
CS376_RS02535	495452..495817	+	366	WP_094031521.1	holo-ACP synthase	-
CS376_RS02540	495982..496989	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
CS376_RS02545	497106..498275	+	1170	WP_025284272.1	alanine racemase	-
CS376_RS02550	498395..498676	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CS376_RS02555	498682..499032	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CS376_RS02560	499150..499971	+	822	WP_003156182.1	STAS domain-containing protein	-
CS376_RS02565	499976..500341	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CS376_RS02570	500344..500745	+	402	WP_099566419.1	anti-sigma regulatory factor	-
CS376_RS02575	500757..501764	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
CS376_RS02580	501828..502157	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CS376_RS02585	502154..502636	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
CS376_RS02590	502602..503390	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CS376_RS02595	503390..503992	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T87087 WP_003156187.1 NZ_CP024203:498682-499032 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T87087 NZ_CP024203:498682-499032 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT87087 WP_003156188.1 NZ_CP024203:498395-498676 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT87087 NZ_CP024203:498395-498676 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5