Detailed information of TA system TA087073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4014638..4015257
• Replicon: chromosome
• Accession: NZ_CP024191
• Organism: Klebsiella pneumoniae isolate KSB1_5D

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: CS526_RS20270
• Protein ID: WP_002892050.1
• Coordinates: 4015039..4015257 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CS526_RS20265
• Protein ID: WP_099595242.1
• Coordinates: 4014638..4015012 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS526_RS20255	4009790..4010983	+	1194	WP_002892072.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
CS526_RS20260	4011006..4014152	+	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
CS526_RS20265	4014638..4015012	+	375	WP_099595242.1	Hha toxicity modulator TomB	Antitoxin
CS526_RS20270	4015039..4015257	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
CS526_RS20275	4015416..4015982	+	567	WP_002892030.1	maltose O-acetyltransferase	-
CS526_RS30460	4015954..4016094	-	141	WP_004147370.1	hypothetical protein	-
CS526_RS20280	4016115..4016585	+	471	WP_002892026.1	YlaC family protein	-
CS526_RS20285	4016560..4018011	-	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
CS526_RS20290	4018112..4018810	+	699	WP_002892021.1	GNAT family N-acetyltransferase	-
CS526_RS20295	4018807..4018947	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
CS526_RS20300	4018947..4019210	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T87073 WP_002892050.1 NZ_CP024191:4015039-4015257 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T87073 NZ_CP024191:4015039-4015257 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14402.08 Da
• Isoelectric Point: 4.8989

>AT87073 WP_099595242.1 NZ_CP024191:4014638-4015012 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFAFNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT87073 NZ_CP024191:4014638-4015012 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTTTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2