Detailed information of TA system TA087053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 3306382..3306913
• Replicon: chromosome
• Accession: NZ_CP024175
• Organism: Bordetella bronchiseptica strain A345

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: CS343_RS15630
• Protein ID: WP_003816744.1
• Coordinates: 3306382..3306648 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: CS343_RS15635
• Protein ID: WP_003816743.1
• Coordinates: 3306635..3306913 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS343_RS15605	3301617..3302327	-	711	WP_003816747.1	aquaporin Z	-
CS343_RS15610	3302435..3303067	-	633	WP_003812619.1	PAS domain-containing protein	-
CS343_RS15615	3303464..3304342	+	879	WP_003816745.1	tripartite tricarboxylate transporter substrate binding protein	-
CS343_RS15620	3304404..3305946	+	1543	Protein_3073	M81 family metallopeptidase	-
CS343_RS15625	3306074..3306264	+	191	Protein_3074	IS481 family transposase	-
CS343_RS15630	3306382..3306648	+	267	WP_003816744.1	BrnT family toxin	Toxin
CS343_RS15635	3306635..3306913	+	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
CS343_RS15645	3307241..3307897	-	657	WP_015041647.1	phosphoribosylanthranilate isomerase	-
CS343_RS15650	3307912..3309579	-	1668	WP_003816741.1	TRAP transporter large permease subunit	-
CS343_RS15655	3309582..3310211	-	630	WP_010930386.1	TRAP transporter small permease subunit	-
CS343_RS15660	3310423..3311517	-	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10032.44 Da
• Isoelectric Point: 9.4798

>T87053 WP_003816744.1 NZ_CP024175:3306382-3306648 [Bordetella bronchiseptica]
MDITYDPAKNDKNIADRGLSFELVRGFEWASALVVEDARQAYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T87053 NZ_CP024175:3306382-3306648 [Bordetella bronchiseptica]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGCGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATGCCCGCCAGGCCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT87053 WP_003816743.1 NZ_CP024175:3306635-3306913 [Bordetella bronchiseptica]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT87053 NZ_CP024175:3306635-3306913 [Bordetella bronchiseptica]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCTGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Antitoxin

• AlphaFold DB: K0MF02