Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 33146..33789 Replicon plasmid p14EC047c
Accession NZ_CP024158
Organism Escherichia coli strain 14EC047

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag CR540_RS26695 Protein ID WP_001034044.1
Coordinates 33146..33562 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag CR540_RS26700 Protein ID WP_001261286.1
Coordinates 33559..33789 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR540_RS26665 (CR540_28035) 28515..28862 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
CR540_RS26670 (CR540_28040) 28984..29262 - 279 WP_001341328.1 transposase -
CR540_RS26675 (CR540_28050) 29463..29723 + 261 Protein_29 transposase -
CR540_RS26680 (CR540_28060) 29779..30476 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
CR540_RS26685 (CR540_28065) 30501..31522 - 1022 Protein_31 DNA helicase UvrD -
CR540_RS26690 (CR540_28070) 31507..33071 - 1565 Protein_32 AAA family ATPase -
CR540_RS26695 (CR540_28075) 33146..33562 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
CR540_RS26700 (CR540_28080) 33559..33789 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CR540_RS26710 (CR540_28085) 34393..34743 + 351 WP_000493379.1 hypothetical protein -
CR540_RS26715 (CR540_28090) 34787..35476 + 690 WP_089583722.1 hypothetical protein -
CR540_RS26720 (CR540_28095) 35473..36264 + 792 WP_000016493.1 site-specific integrase -
CR540_RS26725 (CR540_28100) 36442..36795 + 354 WP_000864812.1 colicin M immunity protein -
CR540_RS26730 (CR540_28105) 36845..37660 - 816 WP_001312845.1 lipid II-degrading bacteriocin colicin M -
CR540_RS26740 (CR540_28115) 37904..38431 + 528 WP_000203272.1 colicin B immunity protein -
CR540_RS26745 (CR540_28120) 38449..38616 - 168 Protein_41 colicin-B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 17996..29750 11754
- inside Non-Mobilizable plasmid sitABCD stcE / stcE / stcE / exeE / exeG / nleA/espI / katA 1..106324 106324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T87014 WP_001034044.1 NZ_CP024158:c33562-33146 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T87014 NZ_CP024158:c33562-33146 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT87014 WP_001261286.1 NZ_CP024158:c33789-33559 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT87014 NZ_CP024158:c33789-33559 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References