Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 20140..20675 Replicon plasmid p14EC047c
Accession NZ_CP024158
Organism Escherichia coli strain 14EC047

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag CR540_RS26645 Protein ID WP_000222766.1
Coordinates 20388..20675 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag CR540_RS26640 Protein ID WP_001132900.1
Coordinates 20140..20391 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR540_RS26585 (CR540_27965) 15168..15883 + 716 Protein_14 A24 family peptidase -
CR540_RS26590 (CR540_27970) 15977..16378 + 402 WP_001222320.1 GspS family T2SS pilot lipoprotein variant EptO -
CR540_RS26595 16504..16668 + 165 Protein_16 IS3 family transposase -
CR540_RS27965 (CR540_27975) 16622..16846 - 225 WP_001368889.1 transposase -
CR540_RS26605 (CR540_27980) 16967..17260 + 294 Protein_18 hypothetical protein -
CR540_RS26610 (CR540_27985) 17489..17742 + 254 Protein_19 replication regulatory protein RepA -
CR540_RS27970 17765..17872 + 108 Protein_20 incFII family plasmid replication initiator RepA -
CR540_RS26640 (CR540_28010) 20140..20391 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CR540_RS26645 (CR540_28015) 20388..20675 + 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD stcE / stcE / stcE / exeE / exeG / nleA/espI / katA 1..106324 106324
- inside IScluster/Tn - - 17996..29750 11754


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T87013 WP_000222766.1 NZ_CP024158:20388-20675 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T87013 NZ_CP024158:20388-20675 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT87013 WP_001132900.1 NZ_CP024158:20140-20391 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT87013 NZ_CP024158:20140-20391 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References