Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 64162..64687 Replicon plasmid p14EC047b
Accession NZ_CP024157
Organism Escherichia coli strain 14EC047

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag CR540_RS26310 Protein ID WP_134894997.1
Coordinates 64382..64687 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CR540_RS26305 Protein ID WP_000813634.1
Coordinates 64162..64380 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR540_RS26270 (59326) 59326..60819 - 1494 WP_015344975.1 IS91-like element ISVsa3 family transposase -
CR540_RS26275 (61030) 61030..61254 - 225 WP_000743213.1 hypothetical protein -
CR540_RS26280 (61251) 61251..61988 - 738 WP_001366550.1 recombinase family protein -
CR540_RS27370 (62474) 62474..62614 - 141 WP_001044210.1 hypothetical protein -
CR540_RS26290 (62620) 62620..63324 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
CR540_RS26295 (63361) 63361..63603 - 243 WP_225288543.1 hypothetical protein -
CR540_RS26305 (64162) 64162..64380 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CR540_RS26310 (64382) 64382..64687 + 306 WP_134894997.1 type II toxin-antitoxin system toxin CcdB Toxin
CR540_RS26315 (64688) 64688..65496 + 809 Protein_74 site-specific integrase -
CR540_RS26320 (65634) 65634..65909 - 276 WP_000239529.1 hypothetical protein -
CR540_RS26325 (65903) 65903..66547 - 645 WP_000633913.1 division plane positioning ATPase MipZ -
CR540_RS26330 (66776) 66776..67746 + 971 Protein_77 plasmid segregation protein ParM -
CR540_RS26335 (67751) 67751..68143 + 393 WP_000340829.1 plasmid partitioning/stability family protein -
CR540_RS26340 (68148) 68148..69417 - 1270 Protein_79 Y-family DNA polymerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / tet(A) / floR - 1..88736 88736


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11761.60 Da        Isoelectric Point: 7.2015

>T87012 WP_134894997.1 NZ_CP024157:64382-64687 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQRDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T87012 NZ_CP024157:64382-64687 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGAGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT87012 WP_000813634.1 NZ_CP024157:64162-64380 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT87012 NZ_CP024157:64162-64380 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References