Detailed information of TA system TA087010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4326504..4327080
• Replicon: chromosome
• Accession: NZ_CP024155
• Organism: Escherichia coli strain 14EC047

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: CR540_RS22000
• Protein ID: WP_001297643.1
• Coordinates: 4326793..4327080 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: CR540_RS21995
• Protein ID: WP_101979790.1
• Coordinates: 4326504..4326806 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR540_RS21980 (4323100)	4323100..4325250	+	2151	WP_001298933.1	pyruvate/proton symporter BtsT	-
CR540_RS21985 (4325300)	4325300..4325503	+	204	WP_000467859.1	YbdD/YjiX family protein	-
CR540_RS21990 (4325514)	4325514..4326470	+	957	WP_001295525.1	GTPase	-
CR540_RS21995 (4326504)	4326504..4326806	-	303	WP_101979790.1	BrnA antitoxin family protein	Antitoxin
CR540_RS22000 (4326793)	4326793..4327080	-	288	WP_001297643.1	BrnT family toxin	Toxin
CR540_RS22005 (4327279)	4327279..4327970	-	692	Protein_4261	IS1 family transposase	-
CR540_RS22010 (4328027)	4328027..4328122	+	96	Protein_4262	dienelactone hydrolase family protein	-
CR540_RS22015 (4328268)	4328268..4329500	+	1233	WP_134894987.1	multidrug efflux MFS transporter MdtM	-
CR540_RS22020 (4329541)	4329541..4330821	+	1281	WP_001037404.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T87010 WP_001297643.1 NZ_CP024155:c4327080-4326793 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T87010 NZ_CP024155:c4327080-4326793 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11536.06 Da
• Isoelectric Point: 9.7969

>AT87010 WP_101979790.1 NZ_CP024155:c4326806-4326504 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLKAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT87010 NZ_CP024155:c4326806-4326504 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAAGGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9