Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3816661..3817279 Replicon chromosome
Accession NZ_CP024155
Organism Escherichia coli strain 14EC047

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CR540_RS19580 Protein ID WP_001291435.1
Coordinates 3817061..3817279 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CR540_RS19575 Protein ID WP_000344800.1
Coordinates 3816661..3817035 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR540_RS19565 (3811750) 3811750..3812943 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CR540_RS19570 (3812966) 3812966..3816115 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
CR540_RS19575 (3816661) 3816661..3817035 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CR540_RS19580 (3817061) 3817061..3817279 + 219 WP_001291435.1 HHA domain-containing protein Toxin
CR540_RS19585 (3817451) 3817451..3818002 + 552 WP_000102568.1 maltose O-acetyltransferase -
CR540_RS19590 (3818118) 3818118..3818588 + 471 WP_000136192.1 YlaC family protein -
CR540_RS19595 (3818752) 3818752..3820302 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CR540_RS19600 (3820344) 3820344..3820697 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CR540_RS19610 (3821076) 3821076..3821387 + 312 WP_000409911.1 MGMT family protein -
CR540_RS19615 (3821418) 3821418..3821990 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87008 WP_001291435.1 NZ_CP024155:3817061-3817279 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87008 NZ_CP024155:3817061-3817279 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT87008 WP_000344800.1 NZ_CP024155:3816661-3817035 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87008 NZ_CP024155:3816661-3817035 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References