Detailed information of TA system TA086997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2410467..2411105
• Replicon: chromosome
• Accession: NZ_CP024155
• Organism: Escherichia coli strain 14EC047

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: CR540_RS12250
• Protein ID: WP_000813794.1
• Coordinates: 2410467..2410643 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CR540_RS12255
• Protein ID: WP_001270286.1
• Coordinates: 2410689..2411105 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR540_RS12230 (2406086)	2406086..2407300	-	1215	WP_001349372.1	BenE family transporter YdcO	-
CR540_RS12235 (2407353)	2407353..2407889	+	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
CR540_RS12240 (2407962)	2407962..2409923	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CR540_RS12245 (2410015)	2410015..2410245	-	231	WP_000494244.1	YncJ family protein	-
CR540_RS12250 (2410467)	2410467..2410643	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CR540_RS12255 (2410689)	2410689..2411105	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CR540_RS12260 (2411184)	2411184..2412590	+	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
CR540_RS12265 (2412835)	2412835..2413980	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
CR540_RS12270 (2413998)	2413998..2415011	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
CR540_RS12275 (2415012)	2415012..2415953	+	942	WP_001251319.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2389946..2415011	25065
	-	flank	IS/Tn	-	-	2404898..2406046	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T86997 WP_000813794.1 NZ_CP024155:2410467-2410643 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T86997 NZ_CP024155:2410467-2410643 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT86997 WP_001270286.1 NZ_CP024155:2410689-2411105 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT86997 NZ_CP024155:2410689-2411105 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB