Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 920633..921287 Replicon chromosome
Accession NZ_CP024155
Organism Escherichia coli strain 14EC047

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag CR540_RS04545 Protein ID WP_000244772.1
Coordinates 920880..921287 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CR540_RS04540 Protein ID WP_000354046.1
Coordinates 920633..920899 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR540_RS04515 (915921) 915921..916664 + 744 WP_000951964.1 SDR family oxidoreductase -
CR540_RS04520 (916721) 916721..918154 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
CR540_RS04525 (918199) 918199..918510 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
CR540_RS04530 (918674) 918674..919333 + 660 WP_000250274.1 hemolysin III family protein -
CR540_RS04535 (919410) 919410..920390 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CR540_RS04540 (920633) 920633..920899 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CR540_RS04545 (920880) 920880..921287 + 408 WP_000244772.1 protein YgfX Toxin
CR540_RS04550 (921327) 921327..921848 - 522 WP_001055874.1 flavodoxin FldB -
CR540_RS04555 (921960) 921960..922856 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CR540_RS04560 (922881) 922881..923591 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CR540_RS04565 (923597) 923597..925330 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T86989 WP_000244772.1 NZ_CP024155:920880-921287 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T86989 NZ_CP024155:920880-921287 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT86989 WP_000354046.1 NZ_CP024155:920633-920899 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT86989 NZ_CP024155:920633-920899 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References