Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 729440..730275 Replicon chromosome
Accession NZ_CP024155
Organism Escherichia coli strain 14EC047

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7W3AJH4
Locus tag CR540_RS03595 Protein ID WP_000854721.1
Coordinates 729898..730275 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2U9LH69
Locus tag CR540_RS03590 Protein ID WP_074463760.1
Coordinates 729440..729808 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR540_RS03570 (727166) 727166..727984 + 819 WP_134894793.1 DUF932 domain-containing protein -
CR540_RS03575 (728076) 728076..728561 + 486 WP_063270646.1 antirestriction protein -
CR540_RS03580 (728577) 728577..729053 + 477 WP_001384029.1 RadC family protein -
CR540_RS03585 (729140) 729140..729361 + 222 WP_000692176.1 DUF987 domain-containing protein -
CR540_RS03590 (729440) 729440..729808 + 369 WP_074463760.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CR540_RS03595 (729898) 729898..730275 + 378 WP_000854721.1 TA system toxin CbtA family protein Toxin
CR540_RS27450 (730272) 730272..730759 + 488 Protein_700 DUF5983 family protein -
CR540_RS03610 (730779) 730779..730976 + 198 WP_000445281.1 DUF957 domain-containing protein -
CR540_RS03615 (731061) 731061..731906 + 846 WP_134894795.1 DUF4942 domain-containing protein -
CR540_RS03625 (732206) 732206..732712 + 507 WP_000228937.1 G/U mismatch-specific DNA glycosylase -
CR540_RS03630 (732791) 732791..734632 - 1842 WP_000437371.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14089.12 Da        Isoelectric Point: 8.2904

>T86987 WP_000854721.1 NZ_CP024155:729898-730275 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T86987 NZ_CP024155:729898-730275 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTATC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13626.39 Da        Isoelectric Point: 6.3159

>AT86987 WP_074463760.1 NZ_CP024155:729440-729808 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT86987 NZ_CP024155:729440-729808 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3AJH4

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LH69

References