Detailed information of TA system TA086985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 75831..76390
• Replicon: plasmid p14EC033g
• Accession: NZ_CP024154
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: CR539_RS27780
• Protein ID: WP_000421257.1
• Coordinates: 76115..76390 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: CR539_RS27775
• Protein ID: WP_001178087.1
• Coordinates: 75831..76115 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS27730	71020..71640	-	621	WP_000176497.1	DUF1449 family protein	-
CR539_RS27740	71907..72572	-	666	WP_101969604.1	traC	-
CR539_RS27745	72713..73354	-	642	WP_000335849.1	transcription termination factor NusG	-
CR539_RS27755	74056..74928	+	873	WP_062860909.1	incFII family plasmid replication initiator RepA	-
CR539_RS27775	75831..76115	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
CR539_RS27780	76115..76390	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR539_RS27785	76498..77195	-	698	Protein_86	IS1 family transposase	-
CR539_RS27795	77273..77554	+	282	WP_001105066.1	hypothetical protein	-
CR539_RS27805	77745..78011	-	267	WP_016237857.1	hypothetical protein	-
CR539_RS27810	78149..78751	-	603	WP_000517694.1	hypothetical protein	-
CR539_RS27820	79105..80451	+	1347	WP_001132021.1	hypothetical protein	-
CR539_RS28355	80978..81280	-	303	WP_001245884.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..84404	84404

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T86985 WP_000421257.1 NZ_CP024154:76115-76390 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T86985 NZ_CP024154:76115-76390 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT86985 WP_001178087.1 NZ_CP024154:75831-76115 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT86985 NZ_CP024154:75831-76115 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCTCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625