Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 54475..54997 Replicon plasmid p14EC033f
Accession NZ_CP024153
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag CR539_RS27060 Protein ID WP_000220561.1
Coordinates 54716..54997 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag CR539_RS27055 Protein ID WP_000121743.1
Coordinates 54475..54726 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS27040 50528..51532 + 1005 WP_000427620.1 IS110-like element IS4321 family transposase -
CR539_RS27045 51920..52624 - 705 WP_032193599.1 replication initiation protein -
CR539_RS27050 52654..53358 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
CR539_RS27055 54475..54726 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
CR539_RS27060 54716..54997 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR539_RS28150 55236..55328 - 93 Protein_68 IS6 family transposase -
CR539_RS27070 55494..56693 - 1200 WP_000948429.1 IS91 family transposase -
CR539_RS27075 56703..56891 - 189 WP_000957857.1 hypothetical protein -
CR539_RS27080 57011..58441 + 1431 Protein_71 IS4-like element IS50R family transposase -
CR539_RS27085 58470..59264 + 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
CR539_RS27090 59285..59509 + 225 WP_101969594.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul3 / tet(A) / qnrS2 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / dfrA12 / aadA2 / aph(3')-IIa / oqxB / oqxA - 1..98181 98181
- inside IScluster/Tn sul3 / tet(A) / qnrS2 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / dfrA12 / aadA2 / aph(3')-IIa / oqxB / oqxA - 9655..66241 56586


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T86984 WP_000220561.1 NZ_CP024153:54716-54997 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T86984 NZ_CP024153:54716-54997 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT86984 WP_000121743.1 NZ_CP024153:54475-54726 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT86984 NZ_CP024153:54475-54726 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References