Detailed information of TA system TA086983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 68413..68972
• Replicon: plasmid p14EC033e
• Accession: NZ_CP024152
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: CR539_RS26515
• Protein ID: WP_000421259.1
• Coordinates: 68413..68688 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: CR539_RS26520
• Protein ID: WP_001178087.1
• Coordinates: 68688..68972 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS26485	64716..66062	-	1347	WP_001132032.1	hypothetical protein	-
CR539_RS26495	66416..67018	+	603	WP_000517694.1	hypothetical protein	-
CR539_RS26500	67074..67568	+	495	WP_015058884.1	hypothetical protein	-
CR539_RS26505	67568..67834	+	267	WP_000627832.1	hypothetical protein	-
CR539_RS26510	68026..68307	-	282	WP_001105066.1	hypothetical protein	-
CR539_RS26515	68413..68688	-	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR539_RS26520	68688..68972	-	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
CR539_RS26540	69876..70907	-	1032	WP_000907852.1	replication initiation protein	-
CR539_RS26555	71880..72143	+	264	WP_001324596.1	ash family protein	-
CR539_RS26560	72061..72348	+	288	WP_000074849.1	conjugal transfer protein TraA	-
CR539_RS26570	72790..73323	+	534	WP_001372168.1	transcription termination factor NusG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..87351	87351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T86983 WP_000421259.1 NZ_CP024152:c68688-68413 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T86983 NZ_CP024152:c68688-68413 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT86983 WP_001178087.1 NZ_CP024152:c68972-68688 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT86983 NZ_CP024152:c68972-68688 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625