Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 38705..39264 Replicon plasmid p14EC033d
Accession NZ_CP024151
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name relE Uniprot ID A0A1V2T4U7
Locus tag CR539_RS25635 Protein ID WP_063073486.1
Coordinates 38705..38980 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A1V2T5E1
Locus tag CR539_RS25640 Protein ID WP_062894229.1
Coordinates 38980..39264 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS25610 34503..35639 - 1137 WP_033804283.1 porin -
CR539_RS25620 36193..37421 + 1229 WP_167654659.1 IS3 family transposase -
CR539_RS25625 37490..37802 + 313 Protein_35 IS66 family insertion sequence element accessory protein TnpB -
CR539_RS25630 37950..38438 + 489 Protein_36 transposase -
CR539_RS25635 38705..38980 - 276 WP_063073486.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR539_RS25640 38980..39264 - 285 WP_062894229.1 ribbon-helix-helix domain-containing protein Antitoxin
CR539_RS25650 40175..41032 - 858 WP_019842754.1 incFII family plasmid replication initiator RepA -
CR539_RS25655 41025..41507 - 483 WP_074149688.1 hypothetical protein -
CR539_RS25660 41500..41574 - 75 WP_001442103.1 RepA leader peptide Tap -
CR539_RS25670 41806..42063 - 258 WP_000083845.1 replication regulatory protein RepA -
CR539_RS25675 42326..42529 - 204 Protein_43 Hok/Gef family protein -
- 42516..42577 + 62 NuclAT_1 - -
- 42516..42577 + 62 NuclAT_1 - -
- 42516..42577 + 62 NuclAT_1 - -
- 42516..42577 + 62 NuclAT_1 - -
CR539_RS25685 43160..43372 - 213 WP_032084501.1 hypothetical protein -
CR539_RS25690 43506..44066 - 561 WP_063073483.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..97858 97858
- inside IScluster/Tn - - 15566..38438 22872


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10724.40 Da        Isoelectric Point: 9.8136

>T86974 WP_063073486.1 NZ_CP024151:c38980-38705 [Escherichia coli]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIYAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T86974 NZ_CP024151:c38980-38705 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTATGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10486.95 Da        Isoelectric Point: 5.6558

>AT86974 WP_062894229.1 NZ_CP024151:c39264-38980 [Escherichia coli]
MQMKNNTAQVTKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLGTDNPLPVPR

Download         Length: 285 bp

>AT86974 NZ_CP024151:c39264-38980 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGTAACAAAAGTAATTACTGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGGTACTGACAATCCGTTACCGGTGCCTCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1V2T4U7

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2T5E1

References