Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 26671..27196 Replicon plasmid p14EC033d
Accession NZ_CP024151
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CR539_RS25545 Protein ID WP_001159871.1
Coordinates 26671..26976 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CR539_RS25550 Protein ID WP_000813634.1
Coordinates 26978..27196 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS25515 22584..23564 + 981 WP_000019440.1 IS5-like element ISKpn26 family transposase -
CR539_RS25520 23830..24400 - 571 Protein_20 IS3 family transposase -
CR539_RS25525 24453..24953 + 501 Protein_21 IS21-like element IS100 family transposase -
CR539_RS25530 24950..25645 + 696 WP_074440817.1 AAA family ATPase -
CR539_RS25540 25864..26670 - 807 WP_020219156.1 site-specific integrase -
CR539_RS25545 26671..26976 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CR539_RS25550 26978..27196 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CR539_RS25565 28012..28302 - 291 WP_032248853.1 hypothetical protein -
CR539_RS25575 28601..29290 - 690 Protein_27 IS3 family transposase -
CR539_RS25580 29325..30059 - 735 Protein_28 IS66 family transposase -
CR539_RS25585 30123..31237 + 1115 WP_085947756.1 IS3-like element IS3 family transposase -
CR539_RS25590 31428..32125 - 698 Protein_30 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..97858 97858
- inside IScluster/Tn - - 15566..38438 22872


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T86973 WP_001159871.1 NZ_CP024151:c26976-26671 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T86973 NZ_CP024151:c26976-26671 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT86973 WP_000813634.1 NZ_CP024151:c27196-26978 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT86973 NZ_CP024151:c27196-26978 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References